Effects of α-isocubebene (ICB) on cytosolic Ca2+ levels in vascular smooth muscle cells.
After pretreating cells with vehicle or ICB (10 µg/ml), phenylephrine (PE, 1 µM) (A) or norepinephrine (NE, 0.1 µM) (B) was added to induce calcium influx. Tracings are representative of 4–5 independent experiments (A). Bottom panel: Relative fluorescence ratio was presented as the means±SEM of 4–5 independent experiments. **p<0.01