Inhibitory effect of α-isocubebene (ICB) on MLC phosphorylation in phenylephrine (PE) (A)- or norepinephrine (NE) (B)-treated VSMC.
After pretreating cells with vehicle or ICB (10 µg/ml), PE (1 µM) or NE (1 µM) was added to elicit MLC phosphorylation. Blots are representative of 5–6 independent experiments. Densitometric results of Western blot are shown in the bottom panel. The p-MLC to t-MLC ratios were presented as the means±SEM of 5–6 independent experiments. **p<0.01 vs. corresponding value in control. ##p<0.01
