Adenine inhibits secretion of TNF-α and IL-6 from LPS-stimulated RAW264.7 cells.
(A–D) Secretion of TNF-α (A, C) and IL-6 (B, D) into the culture medium was determined 6 h after LPS (100 ng/ml) stimulation from RAW264.7 cells in complete culture media. Adenine (A, B, E) or hypoxanthine (C, D, E) was treated for 30 min before LPS stimulation. (E) The cell viability was measured with MTT assay. Significant difference against a control group in each time point of LPS-stimulation is indicated as *(p<0.05).