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Fig. 5.

DRP1 silencing inhibits hypoxia-induced angiogenic function.

(A) EPCs were transiently induced with DRP1 siRNA for 48 h. Cells were harvested and detected the expression of DRP1 to confirm silencing. DRP1 siRNA dose-dependent transduction showed decreased expression. (B) DRP1 silencing cells were seeded into the upper chamber with or without hypoxia for 6 h. DRP1 silencing cells showed decreased cell migration ability with or without hypoxic condition. (C) DRP1 silencing cells were seeded into Matrigel-coated transwell, and incubated under normoxic or hypoxic condition for 24 h. (D, E) Migrating (D) and Invading cells (E) were quantified using the Image J software. All experiment were conducted in at least triplicates. *p<0.05, **p<0.01. (F) DRP1 silencing attenuated the ability of tube formation in the hypoxic condition. Tube structure was observed at 18 h. (G) Total tube length was measured using the Image J software. All experiment were performed at least triplicates. *p<0.05.

Korean J Physiol Pharmacol 2018;22:203-213 https://doi.org/10.4196/kjpp.2018.22.2.203
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