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Fig. 3.

DRP1-dependent mitochondrial fission in EPCs.

(A, B) EPCs were treated with Mdivi-1 for 24 h with serially diluted Mdivi-1 (0–100 µM) in full (A) or starvation (B) media. Cell viability was analyzed and quantified using the CCK-8 assay. Values were normalized to negative control. All experiments were performed in at least triplicates. **p<0.01, ***p<0.001. (C) Cells were treated with 12.5 µM Mdivi-1, and cultured at 1% O2 for 24 h. Mitochondrial morphology was observed using confocal microscopy. White square indicates magnification, as shown in bottom panels. Scale bar=10 µm. TOM20 was used for mitochondrial staining, and DAPI was used for nuclear staining.

Korean J Physiol Pharmacol 2018;22:203-213
© Korean J Physiol Pharmacol