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Fig. 1.

Hypoxia enhances EPC bioactivities.

(A) Human EPCs were incubated under 1% O2 hypoxic or 20% O2 for 24 h. The migration assay was performed with a Boyden chamber. Cell migration was visualized by microscopy (magnification 200×). (B) Quantification of the number of migrating cells. Values represent mean±SEM. Experiments were performed in triplicates. **p<0.01. (C) Invasion assays was conducted with or without hypoxia in EPCs. (D) Quantification of invading cells. Values represent mean±SEM. Experiments were performed in triplicates. *p<0.05. (E) Representative images of hypoxia-induced tube formation in EPCs. EPCs were cultured on Matrigel GFR under normal or hypoxic conditions for 18 h. (F) Quantification of total tube length was performed using the Image J software. Experiments were conducted in triplicates. **p<0.01, as compared with that of control. (G) EPCs surface marker expression analyzed by flow cytometry.

Korean J Physiol Pharmacol 2018;22:203-213 https://doi.org/10.4196/kjpp.2018.22.2.203
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