Concentration-dependent dual effects of plumbagin on MAs and RAs.
A raw trace of plumbagin dose response (0.1–10 µM) without PhE-pretone in endothelium-intact MA (A, n=5). A representative data of 5 µM plumbagin effect on MA pretreated with 1 µM PhE (B, n=14). Plumbagin concentration response curves (0.1–50 µM) in the MAs pretreated with 1 µM PhE (C). Peak of plumbagin contraction is indicated black and steady state of plumbagin response is indicated red. Numbers of tested vessels are indicated above each symbol of peak response (C). A representative trace of plumbagin dose response (0.1–10 µM) without PhE-pretone in endo-intact RA (D, n=6). The dose response curve of plumbagin without pretone in RAs (E). A representative data of 5 µM plumbagin with pre-contracted with 1 µM PhE-pretone in endothelium-intact RA (F, n=9). Plumbagin concentration response curves (0.1–50 µM) in RAs pretreated with 1 µM PhE (G). Each point represents mean of separate experiments and