Augmentation of DFA contractions by PhE and high K+-induced depolarization.
An original trace of plumbagin dose response (0.1–10 µM) showing no effect without pretone in DFA (A, n=5). A representative trace of 5 µM plumbagin effect with 1 µM PhE pretone in endothelium intact DFA (B, n=11) and in denuded endothelium DFA (C, n=7). A raw trace of 5 µM plumbagin response in DFA with 10 µM PhE (D, n=3). Representative traces of 5 µM plumbagin in endo-intact DFAs pre-contracted with 30 mM potassium (E, n=8) and 80 mM potassium solution instead of PhE (F, n=5). The summarization of 5 µM plumbagin effect in endo-intact or denuded DFAs with various pretone conditions (G). Numbers of tested vessels are indicated above each bar graph.