PM10-induced [Ca2+]i signal is attenuated by inhibition of oxidative pathways.
(A) Changes in [Ca2+]i induced by 50 µg/mL PM10. (B) 50 µg/mL PM10 induced [Ca2+]i signaling in the presence of (A) 5 mM NAC (an anti-oxidant), (C) 10 µM 3-AB (a PARP-1 inhibitor), or (D) 10 µg/mL CLP. Top bars indicate the extracellular solution type applied. Traces shown were obtained from average signal. (E) ΔCa2+ was calculated at the indicated dotted line. Results are presented as mean±SEM. *p values of <0.01 were considered significant.