PM10-induced [Ca2+]i signal is dependent on the PLC/IP3 receptor pathway.
(A) Changes in [Ca2+]i induced by 50 µg/mL PM10 in 1 mM Ca2+ medium. (B) 50 µg/mL PM10-induced [Ca2+]i signals were prevented in MRC5 cells by 20 mM caffeine (an IP3R antagonist). 50 µg/mL (C) PM10-induced [Ca2+]i signaling in the presence of PLC inhibitor 5 µM U73122 or its inactive analog 5 µM U73343. The top bars indicate the types of extracellular solutions applied to cells. Traces shown were obtained from average signal except the co-stimulation with PM10 and U73343. (D) ΔCa2+ was calculated at the indicated dotted line. Results are presented as mean±SEM. *p values of <0.01 were considered significant.
