PM10-induced [Ca2+]i signal by extracellular Ca2+ in human lung fibroblast MRC5 cells.
(A) Changes in [Ca2+]i induced by 50 µg/mL PM10 in 1 mM Ca2+ medium (black line) and in Ca2+-free medium (gray line). Top bars indicate the extracellular solutions applied to MRC5 cells. (B) 50 µg/mL PM10-induced [Ca2+]i signals were completely abolished by 10 µM BAPTA-AM. Traces shown were obtained from average signal except the stimulation with PM10 only. (C) ΔCa2+ was calculated at the indicated dotted line. Results are presented as mean±SEM. *p values of <0.01 were considered significant. (D) Cells were stimulated with the presence of La3+. Traces shown were obtained from average signal except the stimulation with PM10 only. (E) Results are presented as mean±SEM and *p values of <0.01 were considered significant. (F) Analysis of size distribution of dust particles.
