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Fig. 5.

Quercetin regulates apoptosis and cell cycle arrest through the JNK-Foxo3a axis.

(A) Cancer cells were transfected with Foxo3a siRNA or scramble siRNA for control prior to treatment with 20 µM quercetin for 48 h. FACS analysis was applied to measure population of apoptotic cells. Representative images of the flow cytometry analysis are shown (left). Statistic graph presents apoptotic levels (right). (B) Cancer cells were transfected with Foxo3a siRNA or scramble siRNA for control prior to treatment with 20 µM quercetin for 48 h. Then, cells were collected and stained with PI, cell cycle was analyzed by FACS. M1, M2, M3, M4 indicate sub G1, G0/G1, S and G2/M phase, respectively (up). The graph shows the percentage of cells in each phase (down). (C) MDA-MB-231 cells were transiently co-transfected with Foxo3a shRNA and a plasmid containing p53, p21 or GADD45 luciferase reporter for 24 h prior to treatment of quercetin. Luciferase activity was measured after 24 h. (D) MDA-MB-231 cells were transfected with p53, p21 or GADD45 luciferase reporter for 24 h prior to pre-treatment of 1 µM SP600125 and treatment of 20 µM quercetin. Luciferase activity was measured after 24 h. *p<0.05, **p<0.01, ***p<0.001.

Korean J Physiol Pharmacol 2017;21:205-213 https://doi.org/10.4196/kjpp.2017.21.2.205
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