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Fig. 4.

Quercetin increased Foxo3a activity via activation of JNK signaling pathway.

(A) MDA-MB-231 was treated with quercetin and levels of p-ERK, p-38, JNK, p-JNK and Foxo3a at 0~8 h were detected by western blot (left). Densitometric quantification of protein expression from western blot analysis (right). The protein levels were compared to basal level at 0 h (B) MDA-MB-231 cells were pre-treated for 30 mins with SP600125 prior to treatment of quercetin for 3 h. Samples were collected and levels of Foxo3a, JNK and p-JNK were determined by western blot. β-actin was used as loading control. (C) MDA-MB-231 cells were transiently transfected with Foxo3a luciferase reporter for 24 h prior to pre-treatment of SP600125 for 1 h and treatment of 20 µM quercetin for another 24 h. Luciferase assay was used to measure activity of Foxo3a. Relative luciferase activity after normalized with Renilla luciferase reporter is shown as fold change from control. *p<0.05, **p<0.01, ***p<0.001.

Korean J Physiol Pharmacol 2017;21:205-213 https://doi.org/10.4196/kjpp.2017.21.2.205
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