Effects of UTD2 on activation MAPKs. MCF-7 cells were treated with the indicated concentrations of UTD2 for 24 hours, and the cell lysates were assayed for phosphorylation of ERK1/2, p38 and JNK, as described in materials and methods. (A) The phosphorylation of ERK1/2, p38 and JNK in MCF-7 cells treated with UTD2 for 24 hours. (B) Optical density of phosphorylated ERK/total ERK. (C) Optical density of phosphorylated p38/total p38. (D) Optical density of phosphorylated JNK/total JNK. The data are presented as mean±SE of these independent experiments (n=4), *p<0.05 and **p<0.01.
