Effect of UTD2 on the migration of stable cell lines expressing Rac1 mutants. (A) Expression of correct myc-tagged Rac1 mutants in MCF-7 subclones. Constitutively active Rac1-(mRac1V12) and dominant negative Rac1-(mRac1N17) was determined by Western blot using a myc tag antibody. Vector represents vector control. (B) The migration of Rac1 mutant subclones was evaluated. The migration was measured as described in migration assay and cells were preincubated with or without 20 nM of UTD2. The data are presented as mean±SE of these independent experiments (n=4), **p<0.01.
