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Fig. 3.

Effects of compound 4 on LPS-induced NO and PGE2 release. RAW264.7 cells were treated with 0~10 µM of compound in the presence of 100 ng/ml of LPS or with LPS alone for 24 h, and (A) NO and (B) PGE2 release was determined. The results are reported as mean±SEM of three independent experiments in triplicate. Statistical significance is based on the difference when compared with LPS-stimulated cells (**p<0.01, ***p<0.001). Thirty micrograms of protein obtained from each cell lysate was resolved by 10% SDS-PAGE for (C) iNOS and (D) COX-2 determination. β-actin expression is shown as a loading control. The bands were quantified using image analysis software and their relative intensity was expressed as fold against the image of the LPS-stimulated RAW264.7 cells.

Korean J Physiol Pharmacol 2013;17:217-222
© Korean J Physiol Pharmacol