Korean J Physiol Pharmacol 2012 Dec; 16(6): 423-429
Yeon Joo Jung, Eun Cheng Suh, and Kyung Eun Lee
Oxygen/Glucose Deprivation and Reperfusion Cause Modifications of Postsynaptic Morphology and Activity in the CA3 Area of Organotypic Hippocampal Slice Cultures
Department of Pharmacology and Ewha Medical Research Institute, Ewha Womans University School of Medicine, Seoul 158-710, Korea
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Brain ischemia leads to overstimulation of N-methyl-D-aspartate (NMDA) receptors, referred as excitotoxicity, which mediates neuronal cell death. However, less attention has been paid to changes in synaptic activity and morphology that could have an important impact on cell function and survival following ischemic insult. In this study, we investigated the effects of reperfusion after oxygen/glucose deprivation (OGD) not only upon neuronal cell death, but also on ultrastructural and biochemical characteristics of postsynaptic density (PSD) protein, in the stratum lucidum of the CA3 area in organotypic hippocampal slice cultures. After OGD/reperfusion, neurons were found to be damaged; the organelles such as mitochondria, endoplasmic reticulum, dendrites, and synaptic terminals were swollen; and the PSD became thicker and irregular. Ethanolic phosphotungstic acid staining showed that the density of PSD was significantly decreased, and the thickness and length of the PSD were significantly increased in the OGD/reperfusion group compared to the control. The levels of PSD proteins, including PSD-95, NMDA receptor 1, NMDA receptor 2B, and calcium/calmodulin-dependent protein kinase II, were significantly decreased following OGD/reperfusion. These results suggest that OGD/reperfusion induces significant modifications to PSDs in the CA3 area of organotypic hippocampal slice cultures, both morphologically and biochemically, and this may contribute to neuronal cell death and synaptic dysfunction after OGD/reperfusion.
Keyword : Neuronal cell death, NMDAR, OGD/reperfusion, Organotypic hippocampal slice cultures, Postsynaptic density

2010 뱬 Copyright The Korean Journal of Physiology & Pharmacology. All Rights Reserved.
8-17, World Cup buk-ro 5ga-gil, Seogyo-dong, Mapo-gu, Seoul 121-841, Korea
Physiology      Tel: +82-2-568-8026    Fax: +82-2-568-8051    E-mail: kps1710@naver.com
Pharmacology    Tel: +82-2-326-0370    Fax: +82-2-326-0371    E-mail: head@kosphar.org
Powered by INFOrang.co., Ltd