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Original Article

Korean J Physiol Pharmacol 2021; 25(2): 119-129

Published online March 1, 2021 https://doi.org/10.4196/kjpp.2021.25.2.119

Copyright © Korean J Physiol Pharmacol.

Centromere protein U enhances the progression of bladder cancer by promoting mitochondrial ribosomal protein s28 expression

Bei-Bei Liu#, Tao Ma#, Wei Sun, Wu-Yue Gao, Jian-Min Liu, Li-Qiang Li, Wen-Yong Li, Sheng Wang*, and Yuan-Yuan Guo*

Department of Urology, the First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233000, China

Correspondence to:Yuan-Yuan Guo
E-mail: liang83278fu047@163.com
Sheng Wang
E-mail: wangsheng_dr@163.com
#These authors contributed equally to this work.

Received: July 1, 2020; Revised: October 26, 2020; Accepted: November 23, 2020

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Bladder cancer is one of the most common types of cancer. Most gene mutations related to bladder cancer are dominantly acquired gene mutations and are not inherited. Previous comparative transcriptome analysis of urinary bladder cancer and control samples has revealed a set of genes that may play a role in tumor progression. Here we set out to investigate further the expression of two candidate genes, centromere protein U (CENPU) and mitochondrial ribosomal protein s28 (MRPS28) to better understand their role in bladder cancer pathogenesis. Our results confirmed that CENPU is up-regulated in human bladder cancer tissues at mRNA and protein levels. Gain-of-function and loss-of-function studies in T24 human urinary bladder cancer cell line revealed a hierarchical relationship between CENPU and MRPS28 in the regulation of cell viability, migration and invasion activity. CENPU expression was also up-regulated in in vivo nude mice xenograft model of bladder cancer and mice overexpressing CENPU had significantly higher tumor volume. In summary, our findings identify CENPU and MRPS28 in the molecular pathogenesis of bladder cancer and suggest that CENPU enhances the progression of bladder cancer by promoting MRPS28 expression.

Keywords: Bladder cancer, Cell, Centromere, Protein