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Korean J Physiol Pharmacol 2013; 17(6): 531-536

Published online December 30, 2013 https://doi.org/10.4196/kjpp.2013.17.6.531

Copyright © The Korean Journal of Physiology & Pharmacology.

Spontaneous Electrical Activity of Cultured Interstitial Cells of Cajal from Mouse Urinary Bladder

Sun-Ouck Kim2,5, Han-Seong Jeong1,5, Sujeong Jang1, Mei-Jin Wu1,6, Jong Kyu Park3, Han-Yi Jiao4, Jae Yeoul Jun4, and Jong-Seong Park1,5,6

Departments of 1Physiology and 2Urology, Chonnam National University Medical School, Gwangju 501-757, Departments of 3Neurosurgery and 4Physiology, College of Medicine, Chosun University, Gwangju 501-759, 5Research Institute of Medical Sciences, Chonnam National University, 6Center for Creative Biomedical Scientists at Chonnam National Univertisity, Gwangju 501-757, Korea

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Interstitial cells of Cajal (ICCs) from the urinary bladder regulate detrusor smooth muscle activities. We cultured ICCs from the urinary bladder of mice and performed patch clamp and intracellular Ca2+ ([Ca2+]i) imaging to investigate whether cultured ICCs can be a valuable tool for cellular functional studies. The cultured ICCs displayed two types of spontaneous electrical activities which are similar to those recorded in intact bladder tissues. Spontaneous electrical activities of cultured ICCs were nifedipine-sensitive. Carbachol and ATP, both excitatory neurotransmitters in the urinary bladder, depolarized the membrane and increased the frequency of spike potentials. Carbachol increased [Ca2+]i oscillations and basal Ca2+ levels, which were blocked by atropine. These results suggest that cultured ICCs from the urinary bladder retain rhythmic phenotypes similar to the spontaneous electrical activities recorded from the intact urinary bladder. Therefore, we suggest that cultured ICCs from the urinary bladder may be useful for cellular and molecular studies of ICCs.

Keywords: Interstitial cells of Cajal, Spontaneous electrical activities, Urinary bladder