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Original Article

Korean J Physiol Pharmacol 2013; 17(1): 15-21

Published online February 28, 2013 https://doi.org/10.4196/kjpp.2013.17.1.15

Copyright © The Korean Journal of Physiology & Pharmacology.

Influence of Aspirin on Pilocarpine-Induced Epilepsy in Mice

Kyoung Hoon Jeong1, Joo Youn Kim1, Yun-Sik Choi3, Mun-Yong Lee2, and Seong Yun Kim1

1Department of Pharmacology, Cell Death Disease Research Center, 2Department of Anatomy, College of Medicine, The Catholic University of Korea, Seoul 137-701, 3Department of Pharmaceutical Science and Technology, The Catholic University of Daegu, Daegu 712-702, Korea

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Aspirin (acetylsalicylic acid) is one of the most widely used therapeutic agents based on its pharmacological actions, including anti-inflammatory, analgesic, anti-pyretic, and anti-thrombotic effects. In this study, we investigated the effects of aspirin on seizure susceptibility and hippocampal neuropathology following pilocarpine-induced status epilepticus (SE). SE was induced by pilocarpine hydrochloride (280 mg/kg, i.p.) administration in C57BL/6 mice (aged 8 weeks). Aspirin was administered daily (15 mg/kg or 150 mg/kg, i.p.) for 10 days starting 3 days before SE, continuing until 6 days after SE. After pilocarpine injection, SE onset time and mortality were recorded. Neuronal cell death was examined using cresyl violet and Fluoro-Jade staining, and glial responses were observed 7 days post SE using immunohistochemistry. In the aspirin-treated group, the onset time of SE was significantly shortened and mortality was markedly increased compared to the control group. However, in this study, aspirin treatment did not affect SE-induced neuronal cell death or astroglial and microglial responses in the hippocampus. In conclusion, these results suggest that the safety of aspirin should be reevaluated in some patients, especially with neurological disorders such as temporal lobe epilepsy.

Keywords: Aspirin, Hippocampus, Mice, Seizure susceptibility, Status epilepticus