pISSN 1226-4512 eISSN 2093-3827

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Original

Korean J Physiol Pharmacol 2006; 10(1): 13-18

Published online March 2, 2006

Copyright © The Korean Journal of Physiology & Pharmacology.

Changes of CA1 Excitability in Rats after Prenatal Methylazoxymethanol Treatment

Sung-Young Jang, In-Sun Choi, Jin-Hwa Cho, Il-Sung Jang, Maan-Gee Lee1, and Byung-Ju Choi

Departments of Dental Pharmacology, 1Pharmacology, College of Medicine, Kyungpook National University, Daegu 702⁣412, Korea

Abstract

Experimentally induced cortical disorganization exhibits many anatomical features which are characteristic of cortical malformations in children with early-onset epilepsy. We used an immunocytochemical technique and extracellular field potential recordings from the dorsal hippocampus to determine whether the excitability of the CA1 pyramidal cells was enhanced in rats with experimentally induced hippocampal dysplasia. Compared with control rats, the MAM-treated rats displayed a decrease of paired pulse inhibition. When GABAA receptor antagonists were blocked with 10μM bicuculline, the amplitude of the second population spike of the MAM-treated of rats was similar to that of the first population spike, as was in the control rats. The MAM-treated rats had fewer somatostatin and parvalbumin-immunoreactive neurons than the control rats. These results suggest that the enhanced neuronal responsiveness of the in vivo recording of the CA1 in this animal model may involve a reduction of CA1 inhibition.

Keywords: Somatostatin, Parvalbumin, Heterotopia, Epilepsy, Dysplasia, Hippocampus