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Original Article

Korean J Physiol Pharmacol 2020; 24(1): 19-26

Published online January 1, 2020 https://doi.org/10.4196/kjpp.2020.24.1.19

Copyright © The Korean Journal of Physiology & Pharmacology.

Medium- and long-chain triglyceride propofol reduces the activity of acetyl-coenzyme A carboxylase in hepatic lipid metabolism in HepG2 and Huh7 cells

Li-yuan Wang1, Jing Wu2, Ya-fen Gao1, Duo-mao Lin1, and Jun Ma1,*

1Center for Anesthesiology, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, 2North China University of Science and Technology, Tangshan, Hebei 063300, P.R China

Correspondence to:*Jun Ma
E-mail: majun7689@163.com

Received: May 23, 2019; Revised: August 23, 2019; Accepted: September 2, 2019

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Medium- and long-chain triglyceride (MCT/LCT) propofol is widely used as an intravenous anesthetic, especially in the intensive care unit. The present study aimed to assess whether MCT/LCT propofol is safe in the hyperlipidemic population for long-term use. Free fatty acids (FFAs) were used to establish high-fat stimulation of HepG2 and Huh7 cells. Subsequently, these cells were treated with propofol at the concentration of 0, 4, or 8 ?g/ml for 24 and 48 h. The results indicated that the cell viability was notably decreased when the cells were stimulated with 2 mmol/L FFAs and treated with 12 ?g/ml MCT/LCT propofol. Accordingly, we chose 2 mmol/L FFAs along with 4 and 8 ?g/ml MCT/LCT propofol for the subsequent experiments. Four and 8 ?g/ml MCT/LCT propofol inhibited FFA-induced lipid accumulation in the cells and significantly reversed acetyl coenzyme A carboxylase (ACC) activity. In addition, MCT/LCT propofol not only significantly promoted the phosphorylation of AMPK and ACC, but also reversed the FFA-induced decreased phosphorylation of AMPK and ACC. In conclusion, MCT/LCT propofol reverses the negative effects caused by FFAs in HepG2 and Huh7 cells, indicating that MCT/LCT propofol might positively regulate lipid metabolism.

Keywords: Hepatocytes, Liver, Metabolism, Propofol