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Original Article

Korean J Physiol Pharmacol 2019; 23(5): 367-379

Published online September 1, 2019 https://doi.org/10.4196/kjpp.2019.23.5.367

Copyright © The Korean Journal of Physiology & Pharmacology.

Profiling of remote skeletal muscle gene changes resulting from stimulation of atopic dermatitis disease in NC/Nga mouse model

Donghee Lee1, Yelim Seo1, Young-Won Kim1, Seongtae Kim1, Jeongyoon Choi1, Sung-Hee Moon1, Hyemi Bae1, Hui-sok Kim2, Hangyeol Kim2, Jae-Hyun Kim2, Tae-Young Kim2, Eunho Kim2, Suemin Yim2, Inja Lim1, Hyoweon Bang1, Jung-Ha Kim3,*, and Jae-Hong Ko1,*

Departments of 1Physiology and 2Medicine, Chung-Ang University College of Medicine, Seoul 06974, 3Department of Family Medicine, Chung-Ang University Hospital, Chung-Ang University College of Medicine, Seoul 06973, Korea

Correspondence to:*Jae-Hong Ko, E-mail: akdongyi01@cau.ac.kr
*Jung-Ha Kim, E-mail: girlpower219@cau.ac.kr

Received: June 29, 2019; Revised: July 7, 2019; Accepted: July 9, 2019

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Although atopic dermatitis (AD) is known to be a representative skin disorder, it also affects the systemic immune response. In a recent study, myoblasts were shown to be involved in the immune regulation, but the roles of muscle cells in AD are poorly understood. We aimed to identify the relationship between mitochondria and atopy by genome-wide analysis of skeletal muscles in mice. We induced AD-like symptoms using house dust mite (HDM) extract in NC/Nga mice. The transcriptional profiles of the untreated group and HDM-induced AD-like group were analyzed and compared using microarray, differentially expressed gene and functional pathway analyses, and protein interaction network construction. Our microarray analysis demonstrated that immune response-, calcium handling-, and mitochondrial metabolism-related genes were differentially expressed. In the Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology pathway analyses, immune response pathways involved in cytokine interaction, nuclear factor-kappa B, and T-cell receptor signaling, calcium handling pathways, and mitochondria metabolism pathways involved in the citrate cycle were significantly upregulated. In protein interaction network analysis, chemokine family-, muscle contraction process-, and immune response-related genes were identified as hub genes with many interactions. In addition, mitochondrial pathways involved in calcium signaling, cardiac muscle contraction, tricarboxylic acid cycle, oxidation-reduction process, and calcium-mediated signaling were significantly stimulated in KEGG and Gene Ontology analyses. Our results provide a comprehensive understanding of the genome-wide transcriptional changes of HDM-induced AD-like symptoms and the indicated genes that could be used as AD clinical biomarkers.

Keywords: Cytokines, Dermatitis, atopic, Microarray analysis, Mitochondria