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Original Article

Korean J Physiol Pharmacol 2019; 23(5): 335-344

Published online September 1, 2019 https://doi.org/10.4196/kjpp.2019.23.5.335

Copyright © The Korean Journal of Physiology & Pharmacology.

Effects of caloric restriction on the expression of lipocalin-2 and its receptor in the brown adipose tissue of high-fat diet-fed mice

Kyung-Ah Park1, Zhen Jin1, Hyeong Seok An1, Jong Youl Lee1, Eun Ae Jeong1, Eun Bee Choi1, Kyung Eun Kim1, Hyun Joo Shin1, Jung Eun Lee2, and Gu Seob Roh1,*

1Department of Anatomy and Convergence Medical Science, Bio Anti-Aging Medical Research Center, 2Department of Thoracic and Cardiovascular Surgery, Institute of Health Sciences, College of Medicine, Gyeongsang National University, Jinju 52727, Korea

Correspondence to:*Gu Seob Roh, E-mail: anaroh@gnu.ac.kr

Received: May 2, 2019; Revised: June 25, 2019; Accepted: July 9, 2019

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.


Obesity causes inflammation and impairs thermogenic functions in brown adipose tissue (BAT). The adipokine lipocalin 2 (LCN2) has been implicated in inflammation and obesity. Herein, we investigated the protective effects of caloric restriction (CR) on LCN2-mediated inflammation and oxidative stress in the BAT of high-fat diet (HFD)-fed mice. Mice were fed a HFD for 20 weeks and then either continued on the HFD or subjected to CR for the next 12 weeks. CR led to the browning of the white fat-like phenotype in HFD-fed mice. Increased expressions of LCN2 and its receptor in the BAT of HFD-fed mice were significantly attenuated by CR. Additionally, HFD+CR-fed mice had fewer neutrophils and macrophages expressing LCN2 and iron-positive cells than HFD-fed mice. Further, oxidative stress and mitochondrial fission induced by a HFD were also significantly attenuated by CR. Our findings indicate that the protective effects of CR on inflammation and oxidative stress in the BAT of obese mice may be associated with regulation of LCN2.

Keywords: Brown adipose tissue, Caloric restriction, Lipocalin 2, Obesity