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Fig. 4. Far-infrared rays (FIR) activate mitochondrial biogenesis at low glucose concentrations via GLUT3. The control and GLUT3 siRNA groups were treated with bio-active materials coated fabric for 72 h under 25 or 5.5 mM glucose conditions. (A) Mitochondrial membrane potential (ΔΨm) as determined by TMRE (tetramethylrhodamine, ethyl ester) fluorescence and (B) confirmation of mitochondrial mass by measurements of NAO (acridine orange 10-nonyl bromide) fluorescence using confocal microscopy (scale bar = 50 μm). (C) TMRE and (D) NAO region of interest fluorescence intensity per cell area ratio. (E) Pgc-1a expression was performed by reverse transcription polymerase chain reaction. ***p < 0.001 vs. control group; ##p < 0.01 vs. experimental group.
Korean J Physiol Pharmacol 2021;25:167~175
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