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Fig. 2. Overexpression of centromere protein U (CENPU) enhanced the viability, migration, and invasion of T24 cells. (A, B) CENPU mRNA and protein expression of each group was measured by qRT-PCR and Western blotting. (C) The cell viability of each group was measured using the cell counting kit-8 (CCK-8) assay. (D) The cell invasion ability was assessed using Transwell assay, magnification ×100. (E) Cell scratch test was performed to evaluate the cell migration abilities. (F) N-cadherin, Slug, and Snail protein expression in transfected T24 cells of each group determined by Western analysis. *p < 0.05 or **p < 0.01 vs. si-NC group or pcDNA-NC group.
Korean J Physiol Pharmacol 2021;25:119~129
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