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Fig. 5. Differential effects of GSK 2033 on expression of M1 surface markers. Serum-starved THP-1 cells were treated with GSK 2033 (1 μM) for 2 h, followed by stimulation with 27OHChol (2.5 μg/ml) for 48 h. (A) Transcript levels of the CD14, CD80, and CD86 genes were assessed by real-time PCR. Data are expressed as the means ± standard deviation (n = 3 replicates for each group). (B) After immunostaining of CD14, CD80, and CD86 with fluorophore-conjugated Abs, cells were analyzed by flow cytometry. The results are representative of 3 independent experiments. ***p < 0.001 vs. control; **p < 0.01 vs. control; *p < 0.05 vs. control; ###p < 0.001 vs. 27OHChol; #p < 0.05 vs. 27OHChol.
Korean J Physiol Pharmacol 2021;25:111~118
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