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Fig. 4. Suppressive effects of GSK 2033 on M2 surface makers. Following serum-starvation, THP-1 cells were treated with GSK 2033 (1 μM) for 2 h and stimulated with 27OHChol (2.5 μg/ml) for 48 h. (A) Transcript levels of the DC163 and CD206 genes were assessed by real-time PCR. Data are expressed as the means ± standard deviation (n = 3 replicates for each group). (B) After immunostaining of CD163 and CD206 with fluorophore-conjugated Abs, cells were analyzed by flow cytometry. The results are representative of 3 independent experiments. ***p < 0.001 vs. control; ### p < 0.001 vs. 27OHChol.
Korean J Physiol Pharmacol 2021;25:111~118
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