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Fig. 3. Opposite effects of GSK 2033 on CXCL8 and TNF-α expression. Serum-starved THP-1 cells were treated with GSK 2033 (1 μM) for 2 h and stimulated for 48 h with 27OHChol (2.5 μg/ml). (A) Transcript levels of the CXCL8 and TNF-α genes were assessed by real-time PCR. (B) The amounts of CXCL8 and TNF-α proteins secreted in culture media were quantified by ELISA. Data are expressed as the means ± standard deviation (n = 3 replicates for each group). The results are representative of 3 independent experiments. The results are representative of 3 independent experiments. ***p < 0.001 vs. control; ###p < 0.001 vs. 27OHChol.
Korean J Physiol Pharmacol 2021;25:111~118 https://doi.org/10.4196/kjpp.2021.25.2.111
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