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Fig. 2. Differential effects of GSK 2033 on 27OHChol-induced expression of C-C chemokines. After serum-starvation overnight, THP-1 cells were treated with GSK 2033 (1 μM) for 2 h and stimulated with 27OHChol (2.5 μg/ml) for 48 h in RPMI media supplemented with 10% FBS. (A) Levels of CCL2, CCL3, and CCL4 gene transcripts were assessed by real-time PCR. (B) The amounts of CCL2, CCL3, and CC4 proteins secreted in culture media were quantified by ELISA. Data are expressed as the means ± standard deviation (n = 3 replicates for each group). The results are representative of 3 independent experiments. FBS, fetal bovine serum. ***p < 0.001 vs. control; *p < 0.05 vs. control; ###p < 0.001 vs. 27OHChol.
Korean J Physiol Pharmacol 2021;25:111~118
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