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Fig. 1. Inhibitory effects of GSK 2033 on the transcription and protein translation of the LXRα and ABCA1 genes. THP-1 cells were serum-starved overnight and treated with GSK 2033 (1 μM) for 2 h. Cells were further stimulated with 27OHChol (2.5 μg/ml) or TO 901317 (1 μM) for 48 h in RPMI media supplemented with 10% FBS. The levels of LXRα (A) or ABCA1 (B) gene transcripts were assessed by real-time PCR. Data are expressed as the means ± standard deviation (n = 3 replicates for each group). LXRs, liver X receptors; FBS, fetal bovine serum. ***p < 0.001 vs. control; ###p < 0.001 vs. 27OHChol or TO 901317; #p < 0.05 vs. 27OHChol or TO 901317. (C) LXRα and ABCA1 proteins were detected by Western blot analysis. Data represent a representative experiment (from 3 independent experiments).
Korean J Physiol Pharmacol 2021;25:111~118
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